Reprogramming of Human Peripheral Blood Cellsto Induced PluripotentStem Cells
Judith Staerk,1 MeeladM. Dawlaty,1 Qing Gao,1
1The Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA
Cell Stem Cell 7, July 2, 2010
Embryonic stem cells are pluripotentcells derived from the inner cell mass of the developing embryo that have the capacity to differentiate into every cell type of the adult (Evans and Kaufman, 1981; Martin,1981; Martin and Evans, 1975; Thomsonetal., 1998). The generation of patientspecificpluripotentcells is therefore an important goal of regenerative medicine.Amajor step to achieve this was the recent discovery that ectopic expression of defined transcription factors induces pluripotencyin somatic cells (Lowry et al., 2008; Park et al., 2008b; Takahashi et al., 2007; Yu et al., 2007). Until now, the most common source from which to derive human iPSCshas been skin fibroblasts (Lowry et al., 2008; Park et al.,2008a, 2008b; Takahashi et al., 2007; Yu et al., 2009). However, the requirement for skin biopsies and the need to expand fibroblast cells for several passages in vitro represent a hurdle that must be overcome to make iPSCtechnology broadly applicable. Peripheral blood can be utilized as an easily accessible source of patient tissue for reprogramming. Here we derived iPSCsfrom frozen human peripheral blood samples. Some of the iPSCshad rearrangements of the T cell receptor (TCR), indicating that T cells can be reprogrammed to pluripotency.